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The complete probe set used for microarray analysis contained all annotated genes for nitrogen metabolism for each included genome, and in many cases, genes from both nitrate assimilation and dissimilatory nitrate/nitrite reduction pathways were present in the same organism.
Accordingly, incomplete reprogramming of hiPSCs, as demonstrated by this study, could reflect the fact that the cell population used in the microarray analysis contained contaminating subpopulations of cells that have not been completely reprogrammed, and thus give rise to expression signatures of the parental cells [46].
Each sample for microarray analysis contained a pool of whole rosettes (minus the leaf taken for the metabolomic analysis) from three or four plants of approximately equal size.
The microarray analysis contained two additional experimental replicates for each treatment and cultivar, preventing a direct one-to-one comparison between replicates; thus, mean expression (transcript) and abundance (protein) values were computed for each treatment and cultivar prior to regression analysis.
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We performed Affymetrix HGU133A plus 2.0 microarray analysis, containing probes for about 39,000 human transcripts, comparing 9 human pleural mesotheliomas with 4 normal pleural specimens.
We used a neuroblastoma tumor panel for Affymetrix Microarray analysis containing 88 primary neuroblastoma tumor samples of untreated patients of which 87 neuroblastoma tumor samples were also used for CGH analysis and SNP array [ 34].
To identify miRNA signature in SS, we profiled the expression of 28 SS samples (22 patients and 6 follow-up) and six healthy controls (HCs) by the means of microarray analysis containing 470 human miRNAs.
All these data are reflected in Table 2 and according to them, five genes of known function already resolved by microarrays themselves have been confirmed by qRT-PCR and 16 clones not directly resolved by microarray analysis contain at least one differentially regulated gene.
The microarray-based transcriptome analysis contained in this study has revealed that eleven amastin genes are up-regulated under TPS, ten out of these under TS but none under PS.
A total of 194 breast tumors were analyzed by clustering analysis using a modified version of the 'SAM264' gene list [ 18]; the 'SAM264' gene set is the set of genes that were associated with survival as identified using a Significance Analysis of Microarray analysis and contained 10 of the 21 genes sequenced.
In another elegant work, ExMVs were demonstrated by microarray analysis to contain mRNAs for 1300 genes, and after ExMV-mediated transfer of murine mRNA to human cells, murine-derived proteins were found to be expressed in recipient human cells [14].
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