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The second group of samples for microarray analysis consisting of 3 CTGF-treated and 3 corresponding control ovaries was run, pre-processed and analyzed post factum, separately from the rest of the samples as a result of a discovery from network analysis.
More recently, the miRNA expression patterns in non-tumor liver tissue in HCC patients without multicentric recurrence in more than 3 years and those with multicentric recurrence within 3 years after hepatectomy were compared using a miRNA microarray analysis consisting of 955 probes.
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The initial cohort for microarray analysis consisted of 29 early-stage, invasive ductal carcinoma breast tumour specimens.
The microarray data analysis consisted of the following steps: 1. quantile method normalization, 2. global clustering and PCA-analysis, 3. fitting the data to a linear model, 4. detection of differential gene expression and 5. over-representation analysis of differentially expressed genes.
The microarray data analysis consists of the following steps: 1. between-array normalization, 2. global clustering and PCA-analysis, 3. fitting the data to a linear model, 4. detection of differential gene expression and 5. over-representation analysis of differentially expressed genes.
The basic requirements of an analysis consist of a genome annotation (i.e., Genbank or EMBL) and a collection of microarray data.
Our analysis consisted of four scenarios.
Each analysis consisted of four chromatography steps.
Analysis consisted of descriptive statistics.
Each oligonucleotide probe for microarray analysis was designed to consist of sequences of two adjacent exons to specifically interrogate the cDNA from corresponding mRNA sequence, but not the corresponding gene sequences or cDNA from unprocessed RNA.
Genome-wide microarray analysis identified a cardiopoietic profile consisting of 16,721 differentially expressed transcripts (4,163 up- and 12,558 down-regulated) that distinguished the pro-cardiac cytotype from the pluripotent ground state (Figure 1B).
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