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Interestingly, the microarray analysis by Blenkiron et al. revealed that miR-33a is often lost in human breast cancer (Blenkiron et al., 2007).
Microarray analysis by Blenkiron et al. showed that miR-33a expression is often lost in human primary breast cancers because of chromosome alterations, indicating that miR-33a may function as a tumor suppressor (Blenkiron et al., 2007).
For example, a microarray analysis by Rabbani et al. [46] has showed that 36 genes appear to be induced in rice under cold stress and that the expression level for several genes reached a maximum after 24 h of cold treatment.
We confirmed the results obtained by microarray analysis by quantitative RT-PCR (real-time PCR) on a limited number of genes.
We simplify microarray analysis by converting the real values in raw data into 3 discrete values: −1 is down, 0 is around the mean value and +1 is up (see Experimental Procedures).
To identify potential downstream target genes of Spt5, we performed microarray analysis by hybridizing zebrafish Affymetrix oligoarrays, which contain over 10,000 protein-coding genes, with biotinylated cRNA probes from 24 hpf fogsk8 mutant and WT sibling embryos.
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Subsequent oligo-microarray analysis by Yu and collaborators revealed that CyPA can upregulate the expression of many cytokine and drug resistance-related genes including drug metabolism and drug transport-related genes.
Induction of the pro-apoptotic gene, BAX, in MCF-7 cells by BaP was shown by microarray analysis and by RTqPCR.
Seven genes shown to be differentially expressed were selected from the results obtained by microarray analysis and by proteomic analysis.
Microarray analysis validated by quantitative PCR demonstrated an extensive range of pattern recognition receptor gene expression in resting N9 microglia, including Toll-like receptors, scavenger receptors and lectins.
Our microarray analysis followed by functional annotation clustering revealed statistically significant enrichment related to metabolism and membrane transport and transporters.
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