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For the microarray analysis, bases of the lowest whorl branches from the H- and the C-plants were collected and put into liquid nitrogen at −78°C on September 2, 2011.
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Seven paired patient samples were selected for microarray analysis based on the quality of RNA and presence of intestinal metaplasia without gastric mucosa or dysplasia.
We have developed a web tool for pangenome microarray analysis based on our PanCGH algorithm.
PanCGHweb is a web tool for pangenomic microarray analysis based on PanCGH algorithm [21].
However, DNA microarray analysis based on steady-state mRNA (total mRNA) does not always correspond to transcriptional activation or repression.
The genes that were selected from the microarray analysis, based on known localization and function, were confirmed on RNA and protein levels.
First, to select 5 fish per treatment at 170 dph for further microarray analysis based on 2DCt cyp19a1a qRT-PCR values after a two-step clustering analysis (see statistical section below).
To elucidate molecular defence responses activated by BABA in potato, a genome-wide microarray analysis based on the recently released Solanum tuberosum group phureja genome [ 15] was performed in combination with label-free quantitative proteomic analysis of the apoplast secretome.
Out of ten plants, three plants were chosen for the microarray analysis based on typical, average phenotype of HC-Pro plants (see Figure 1) and on average transgene HC-Pro expression (Additional file 1).
In this study, we succeeded in obtaining a large-scale catalog of marking-specific genes and stage-specific genes from the swallowtail butterfly larvae by microarray analysis based on EST datasets.
Nevertheless, absolute qPCR has the potential to extend gene expression analysis beyond what is possible with microarray analysis, based upon the innate capability to overcome two of the greatest limitations of microarray quantification, which are limited sensitivity and lack of absolute scale [ 4].
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