Finally, cDNA microarray analysis of approximately 7,100 cDNAs identified a number of differentially expressed genes in the 436.1 cells.
Use of the Affymetrix microarray analysis revealed approximately 10% of the whole human genome effectively expressed in DCs (data not shown).
The microarray analysis of approximately 2,600 different transcripts revealed a total of 120 regulated genes divided into 3 patterns, down regulated from pre-ad II to adult T1 (group 3), up-regulated from pre-ad II to T1 and then down regulated (group 2), and up-regulated from T2-T6 (group 1).
For the microarray transcription analysis, approximately 100 salivary glands in triplicate were dissected 2 h after blood feeding (from both blood-fed and unfed mosquitoes).
Microarray analysis revealed that approximately 17% of Arabidopsis miRNAs are up-regulated in response to low temperature at early stages of cold treatment [ 9].
Both qRT-PCR and microarray analysis showed an approximately 8-fold increase in transcript abundance after 6 h of NaCl treatment, with further accumulation to a 32-fold increase by 48 h.
The data in the microarray analysis showed an approximately 6.2 and 3.2 reduction in syndecan-2 expression compared with the control in the dragonfly and removal of salamander treatment groups, respectively.
Total RNA isolated for microarray analysis was diluted to approximately 200 ng/uL.
Using two-color microarray-based gene expression analysis (Agilent, Santa Clara, CA) with Cluster 3.0 and Treeview expression microarray analysis software, we identified approximately 1616 genes with altered expression level of more than 1.5-fold after Sall2 transfection compared with the vector control group among 44 000 probes (Fig. 1).
Dittwald and colleagues [ 30] identified 2,129 known pathogenic LCR-mediated CNVs from 25,144 individuals sent for chromosomal microarray analysis at a diagnostic lab (approximately 8.5%).
