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NGLs were arrayed robotically, and the microarray analyses with anti-glucan antibodies were performed essentially as described [62].
In contrast, by connecting sub-networks of genes identified in microarray analyses, with proteins known to bind the CTD, we were able to form putative connections between phenotypic changes and the specific CTD alterations introduced.
For example, for prostate cancer, CDIP contains 119 different microarray analyses with over 850 tumor and normal samples.
JSM established the drug resistance culture model and performed the methylation microarray analyses, with supervision from PY and TH-MH.
Consistently, most of the regulated genes found in the microarray analyses with different CcpA induction levels were downregulated.
Similar modulation patterns were found for the expression of the eight genes comparing microarray analyses with qRT-PCR (Supplementary Figure 1).
Similar(46)
To gain further insight into nTreg development and function, several DNA-microarray analyses with murine nTreg were performed.
Microarray analyses, performed with RNA from human SCC25 cells treated with 1 α,25(OH)2D3 and cycloheximide, an inhibitor of protein synthesis, revealed 913 regulated genes [ 40].
All differentially expressed genes in microarray analyses, along with known RNAP II-protein interactions downloaded from Thebiogrid.org, were loaded into the Cytoscape program (www.cytoscape.org; [52], [53]).
Technologies applied for host-directed drug discovery include cDNA and siRNA-based microarray analyses combined with pathway-guided data mining [47], [48], [49], [50], [51], loss-of-function screens using aptamers or small oligonucleotides [52], [53], [54], [55], [56], [57], [58], protein array analyses [59] and chemical library screening [60], [61].
Latest generation microarray analyses start with 100 ng total RNA that can be isolated from very small amounts of tissue.
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