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For microarray analyses, the wt embryos were collected at 24 hpf, 48 hpf and 72 hpf, snap-frozen in liquid nitrogen and stored at -80°C.
The initial purpose of the present study was to investigate, using microarray analyses, the influence of SCH23390 on transcriptional responses in the rat striatum caused by a single METH injection at 2 and 4 hours after drug administration.
For DNA microarray analyses, the RNA was labeled with GeneChipR One-Cycle Target Labeling and Control Reagents and loaded on to the Affymetrix Human Genome U133 plus 2.0 GeneChip according to the manufacturer's recommendations (Affymetrix, Santa Clara, CA).
For tissue microarray analyses, the chi-square test was used.
For RNAs destined for microarray analyses, the polysomal fractions and non-polysomal fractions were pooled respectively.
To perform DNA microarray analyses the microarray platform GPL6467 has been used [ 29].
Similar(41)
BT collected the data, carried out the initial microarray analyses, interpreted the data and revised the manuscript.
MG performed the microarray analyses and the associated statistics, and drafted the manuscript.
FH performed the microarray analyses and the associated statistics, and drafted the manuscript.
DL performed the microarray analyses and the associated statistics, and drafted the manuscript.
HW performed the microarray analyses, initiated the pathway analysis project and revised the manuscript.
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