While there have been several studies which evaluated LCM-coupled microarray analyses, none have reported the above observations in lung or lung adenocarcinoma [ 11, 12, 15- 18].
Comprehensive evaluation of seed transcripts through microarray analyses have been reported for Arabidopsis [ 34], Medicago truncatula [ 4, 38], barley [ 5, 41], and wheat [ 40].
The BDNF-associated gene is also functionally responsible for type 2 diabetic nephropathy, as determined through previously reported microarray analyses [ 31].
Moreover, recent studies reported microarray analyses which determine whether miRNAs are deregulated in common cardiovascular physiopathological conditions, such as hypertrophic and/or dilated cardiomyopathy, heart failure, or atrial fibrillation [ 12– 17].
Based on our result of microarray analyses and previous reports [ 18– 23], in this study, several genes related to the immunosuppressive properties of MSCs were selected, and these included PTGES and ULBP.
The confirmation rate we observed in our study was similar to those reported in microarray analyses of other polyploid species.
Problems associated with sensitivity and reproducibility have been reported for microarray analyses, even for the ones conducted with manufactured Affymetrix Gene Chips [ 22].
In addition, the comparison of LIF regulated genes identified in our study with those reported in microarray analyses, performed on other cell types or tissue [cardiomyocytes [ 64], neurons [ 65] and uterus [ 66]], shows no overlap except stat3 (summary in Figure 9).
Although both RNAseq and microarray analyses measure the transcriptome, studies report different genes as DE using one method or the other, even when using the exact same samples [ 43– 43].
