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In order to validate the results obtained by microarray analyses, quantitative RT-PCR (qRT-PCR) was performed by random sampling.
In parallel to the microarray analyses, quantitative PCR expression ratios were established by comparing normalised relative expression at each time point after clostridial challenge to that before the challenge (D0 PI) within each treatment group.
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Their brains were quickly removed, striatal and SN/VTA tissues were dissected on ice, snap frozen on dry ice, and stored at −80°C until used in either HPLC, microarray analyses, or quantitative PCR experiments as described below.
Secondary outcome included validation of the results of microarray analyses by quantitative reverse transcription-PCR.
We validated the results of the microarray analyses using quantitative reverse transcriptase-PCR.
Microarray analyses and quantitative real-time polymerase chain reaction analyses show that Eif1a-like genes are expressed specifically in the Zscan4-positive state of ES cells.
We present here results from annotated genes identified through microarray analyses and specifically quantitative PCR analyses of selected molecular biomarkers.
In order to validate our findings with the microarray analyses, we performed quantitative real-time PCR (qPCR) analysis on these four cell lines.
To validate the microarray analyses, we performed quantitative real time RT-PCR of eight selected genes whose expression levels were significantly altered in the microarray experiments at stationary phase (Fig. 3).
In microarray analyses in Senecio allopolyploids, quantitative RT-PCR confirmation rates were also approximately 65% [25], [26].
cDNA samples prepared for microarray analyses were utilized for quantitative real-time PCR (RT-qPCR) validation of global expression analysis.
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