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Global transcriptional profiling with microarray analyses further supports the hypothesis that TSPY exerts pro-growth and proliferative functions in the cell cycle progression of its host cells.
Reinforcing such a link between the two RNA processing events, a more systematic approach utilizing high-throughput RNA-seq and exon-specific microarray analyses further revealed at the global scale that ADAR1 targets alternative splicing [ 37].
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Gene expression and regulation was determined by microarray analyses and further evaluated by bioinformatics tools including pathway and cluster analyses.
The mRNA levels differentially expressed between the treatment groups in microarray analyses were further validated using Real-time PCR.
With the completion of the sequencing of the genomes of human, mouse and rat, the results of microarray analyses can be further extended to comparative analyses of homologous genes of interest.
Data obtained from microarrays analyses were further verified and visualized by semi-quantitative RT-PCR on independent material.
This study provides a foundation for the molecular, biochemical and functional exploration of selected molecules with differential transcription profiles in H. contortus, for further microarray analyses of transcription in different developmental stages of H. contortus, and for an extended functional analysis once the full genome sequence of this nematode is known.
Microarray analyses were used to further understand the role of HLA-B*35 allele.
This was an open-label, single-group pilot trial, which included histopathologic and microarray analyses of skin to further delineate biological response.
Further, microarray analyses of the overcompensating genotype Columbia-4 identified a total of 109 differentially expressed genes between damaged and undamaged plants [ 23], one of which resides within a significant QTL region.
A selection of 12 genes that showed stable expression across the microarrays were analysed further by QRT-PCR (Genes 1-12; Table 2).
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