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Except for the microarray analyses, data are represented as mean values±SEM, with N representing the number of experiments.
Transcripts for all eight genes showed similar patterns by both RT-PCR and microarray analyses (data not shown).
Additionally, no significant amounts of endogenous TSHα mRNA could not be detected by quantitative RT-PCR and microarray analyses (data not shown).
GLAST, encoded by the SCL1A3 gene, was expressed in primate taste buds by microarray analyses, but was not detectable by ISH, while NTPDase2, encoded by the ENTPD2 gene, was not expressed at detectable levels in primate taste buds by microarray analyses (data not shown); thus, we were unable to compare the histological expression pattern of TMEM44 with these gene products in primates.
For gene expression microarray analyses, data were normalized using Robust Multi-array Analysis (RMA) with R-package "affy".
The mRNA abundance of the four major circadian genes: period (per), clock (clk), cycle (cyc) and cryptochrome 1 (cry1) was below threshold detection levels for microarray analyses (data not shown) at the assayed conditions (light on/off time-zone).
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Currently, one major drawback when performing microarray analyses on data derived from dietary intervention studies is the lack of proper statistical tools.
RF contributed to microarray analyses and data processing, bioinformatic analyses, and manuscript preparation and editing.
RSP contributed to microarray analyses and data processing, bioinformatic analyses, and manuscript editing.
AM collected the data, carried out the initial microarray analyses and interpreted the data.
The GEO accession number for the raw data of microarray analyses is GSE20497.
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