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Cytogenetic analyses like G-banded kandotype and chromosomal microarray analyses are often performed to further investigate the chromosome status of a miscarried fetus.
Based on these findings it seems likely that standard microarray analyses are underestimating disease specific gene expression changes.
These approaches are complementary: limma and other conventional microarray analyses are appropriate to detect genes that are differentially expressed in close to every single sample of a group, whereas Messina is optimised to detect differences between groups even if the differential expression is present at a low frequency.
In order to test whether SUMO modification of DRIL1 can modulate its transcriptional activity, we expressed wt, K398R, Kx4R mutants or empty vector in 293T cells and performed genome-wide expression microarray analysis (the raw data of the microarray analyses are available at http://www.ebi.ac.uk/microarray-as/aer/ ae-main[0], accession E-TABM-681).ac.uk/microarray-as/aer/ ae-main[0]
The DNA microarray analyses are described above.
The results of microarray analyses are shown in Figure 3.
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In both experiments, full genome microarray analyses were carried out on RNA extracted from whole animals recovered from the flight.
Subsequently, microarray analyses were validated by RT-PCR and qRT-PCR.
The microarray analyses were performed as described previously (Hanada et al. [2013]) with the exception that two biological replicates were used.
Microarray analyses were performed after one week of Fe deficiency and excess treatment and at this point, plants correspond to a new transcriptomic/metabolic steady state.
Microarray analyses were performed in 3T3-L1 preandpocytes and mature adipocytes treated with 0.1 mg·mL−1 caffeine to identify factors that might be involved in the mechanisms underlying these effects.
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