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Labeled cDNA samples were individually hybridized to the microarray, according to the NimbleGen standard operating procedure.
The labeled cDNA was then hybridized to the Incyte mouse GEM1 cDNA microarray according to their established protocol.
Immunohistochemistry was performed for the tissue microarray according to the protocols described above for the Test Group.
The labeled cDNA was used to perform overnight hybridization to Agilent's rhesus macaque whole genome microarray, according to the manufacturer's instructions [28].
The PAMPs binding assay of rCfLec-1 was performed by the method of PAMPs microarray according to the previous report with modification [29], [30].
Briefly, DNA samples were fluorescently labelled and hybridized to the Agilent 8×15K format microarray according to manufacturer's instructions for the Agilent genomic DNA enzymatic labelling and oligo aCGH hybridisation reagent kits.
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8 µg of cRNA were hybridized to Affymetrix Human Genome U133A microarrays, according to the manufacturer's protocol.
cDNA was prepared and hybridized to MOE 430 2.0 Affymetrix GeneChip® oligonucleotide microarrays, according to standard protocols at the UCLA Facility.
For microarray analyses, one microgram of total RNA of each sample (n = 4) was used to prepare biotinylated fragmented cRNA, which was produced and hybridized to Affymetrix Rat Genome 230 2.0 GeneChip microarrays according to the GeneChip Expression Analysis manual.
Total RNA was prepared using Affymetrix (Santa Clara, CA) microarrays, according to the directions from the manufacturer.
Macroarray hybridisation was performed on BD Clontech ATLAS human 1.2 microarrays according to the manufacturer's instructions.
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