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Sera were also tested for the presence of virus neutralizing antibodies specific for the two influenza viruses using a micro virus neutralization (VN) assay with the viruses that were produced by reverse genetics as described above [40].
Virus-neutralization titers against WNV (strain Eg-101), BAGV (strain Spain/2010), and USUV (strain SAAR1776) were determined by micro virus neutralization test (VNT) as described (6 ).
Neutralizing antibody titers against WNV (strain Spain/2007 GE-1B/b) and Usutu virus (USUV) (SAAR1776) were determined by using a micro virus-neutralization test as described previously (5 ).
Additionally, Vaccine Investigation and Online Information Network (Violin; http://www.violinet.org), provides gene and protein sequences that are affiliated with infection mechanisms of various micro (virus, bacteria, protozoon) and macro (helminth) infectious organisms [ 15].
The samples were screened for neutralizing antibody against the equine WNV strain, Morocco 96 111 (3 ), by using a micro virus-neutralization test in 96-well plates and an adaptation of a described method (5 ).
Titers determined by the micro virus-neutralization test ranged from 18 to 2,630 (Table).The prevalence of WNV neutralizing bodies was significantly higher in cohort B and C participants than in cohort A participants (p<0.01).
When immunized sera were processed for micro-virus neutralization assays using 100 TCID50 of CA/04/09 in Madin-Darby canine kidney (MDCK) cells, administration of the reverse-genetics vaccine demonstrated efficient virus neutralizing activity which correlated comparably well with the vaccine-induced HI responses (data not shown).
Neutralizing antibody titers for WNV (strain Eg101) were determined by using a micro-virus neutralization test as described (6 ).
Specificity of the test was assessed by parallel neutralization against Usutu virus (strain SAAR 1776), a flavivirus found in wild birds that belongs to the same serogroup as WNV, with a panel of sera positive for WNV by micro-virus neutralization test.
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