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All procedures conform to the manufacturer's protocol except for the adaptation of the micro method for fish, described by Bagni et al. (2007).
However, if it is modeled with micro method this volume should be replaced with a large number of small elements consisting of brick and mortar (0.25 × 0.25 × 0.06 m).
Of the screening samples, 84% were later tested for the presence of GAD65, ICA512, and IAA measured by the micro method (mIAA).
CAV-2 hemagglutination inhibition (HI) antibody titers were determined by a micro method with a slight modification (see Additional file 5) [ 14].
The CFT, first described in 1953 [ 3], is widely used in countries struggling with the disease, and a modified "micro method" is common in countries of the European Union [ 2].
The N-acetyl glucosamine released by the chitinase action was then determined using a colorimetric method as described by Reissig et al. [ 52] and adapted for a micro method.
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Considering the size of the sample elemental analysis of the sample in a pellet can be carried out only by micro methods such as SEM-EDX.
Phospholipid phosphorus was determined using sub-micro method [35].
MICs were determined by a micro-method described previously (Schillaci et al. [2005]).
Total nitrogen (N) was determined by the Kjeldahl micro-method using 100 mg of lyophilized biomass (Bremner 1965).
MICs of 5-HCC and of chemically synthesized peptides were determined by a micro-method as previously described (Schillaci et al. 2008; Schillaci et al. 2005).
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