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The HPLC system was composed of an Agilent 1200 capillary nano pump, a binary pump, a thermostated micro injector and a micro switch valve.
Manually defolliculated stage V-VI Xenopus oocytes were injected with 5 ng of cRNA (50 nl at a concentration of 0.1 μg/μl) using an Inject +Matic micro injector (J.
Five-to-ten day old flies were injected with ≤0.1 μl bacterial suspension per fly using a glass capillary connected to a micro injector (TriTech Research, Los Angeles, CA, USA).
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Drugs were microinjected into the epicenter with a micro-injector fixed by stereotaxic apparatus with a rate of 0.5 µl/min.
Oocytes were injected with 50 70 nl of cRNA at a concentration of 1 3 ng/nl using a micro-injector (Drummond Scientific, Broomall, PA).
From these results, the system demonstrated sufficient accuracy to guide the micro-injector from the planned delivery routes into practice.
The laboratory-constructed device is assembled from an array of components including two syringe pumps, a nano-gradient mixing chip, a micro-injector, a diode laser, and a separation capillary column made from a polymer/silica (PEEKsil) tube.
The miniaturized device for immunoaffinity chromatography was constructed from two syringe pumps, a gradient mixing microchip, micro-injector with 250 nL capillary injection loop, a capillary column, and a diode laser-induced fluorescence detector fitted with a fused-silica capillary flow cell.
Then, 20 µL of the ink was dropped on the working electrode by a micro-injector and it was dried at 50 °C for 10 min until the sample formed a film.
We delivered 2 μL IS to the rats in the IS and the IS + AMI groups, and the same volume of saline to the rats in the AMI and CON groups through the cannula using a micro-injector for 21 d.
We injected a total of 90,000 cells (2 µl/site; 15,000 cells/µl) using a micro-electrode (80 µm of diameter) connected to a micro-injector (0.2 µl/min) and to a stereotactic micromanipulator.
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