To further investigate the role of CD146 in BBB integrity, we micro-injected the tracing dye of rhodamine B-dextran (70 kDa) into the vasculature of cd146-MO zebrafish larvae at 3 dpf, when the function of BBB was initially formed.
To further confirm these results, we micro-injected αH6 and αH6m peptides into Jurkat cells (Figure 4F).
In order to directly test whether Pgc protein, rather than its RNA message, is responsible for the decrease in CTD Ser2 phosphorylation in vivo, we micro-injected the anterior pole of stage 3 4 Drosophila embryos with folded Pgc.
In order to test whether the oocyte phenotype of Prep1i/i mice correlates with the increased Hoxb4 mRNA translation, we micro-injected fertilized oocytes from super-ovulated females with either CMV-IRES-GFP or CMV-Hoxb4-IRES-GFP vector and examined their development in culture.
Next, we micro-injected CaMKIIα inhibitor TatCN21 or Tat control (Tatcont) into the IC 25 min from the beginning of the taste consumption in short-trace 3 hr ITI-CTA conditioning, and observed an attenuated CTA memory.
We micro-injected the NMDAR antagonist into the IC 25 min from the beginning of the taste consumption in 1 hr ITI-CTA conditioning with a weak US and observed an attenuated CTA memory, consistent with a previous report in which a strong US was administered (Rosenblum et al., 1997; but also see Ferreira et al., 2002) .
In brief, the micro injection method directly injects nucleic acid into the cytoplasm or nucleus [ 14, 15].
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Herein, we presented a novel materials-sensor integration fabrication strategy: on basis of screen printing (SP) technology and calcination, micro-injecting (MI) was introduced into the fabrication process of sensors, which was named as SPMIC, to obtain In2O3 nanowire-like network directly on the surface of coplanar sensors array by structure replication from sacrificial carbon nanotubes (CNTs).
After injecting micro bubble treatment fluid, it is found that the result is effective.
We exploited this micro-environment by injecting Xenopus kidney epithelial cells transfected to overexpress vascular growth factors.
