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The probe was perfused (5 μl/min) with a Ringer-Acetate solution containing 0.5 mM Ringer-lactate with a 2-ml syringe connected to a micro infusion pump (MAB 140, Microbiotech AB, Stockholm, Sweden).
They injected β-END bilaterally by micro infusion via brain cannulas into several specific rat brain areas and made some striking observations.
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Further, micro-infusion of CGP44532 directly into the ventral tegmental area elevated ICSS thresholds similarly in saline- and nicotine-treated rats.
The catheter was perfused (5 μL/ min) with a Ringer-Acetate solution (Pharmacia & Upjohn, Copenhagen, Denmark) containing 3 mmol glucose and 0.5 mmol lactate from a 2-ml syringe connected to a micro-infusion pump (CMA107, Microdialysis AB, Solna, Sweden).
The device is composed of 1.2 m of fused silica inserted through a 24.6-cm surgical steel cannula designed to fit a standard Leksell® clinical stereotaxic frame and micro-infusion syringe pump.
Viruses were injected over a 10-min period using a 32-gauge syringe needle (Hamilton, Reno, NV) attached to a micro-infusion pump (WPI, Sarasota, FL).
Micro-infusion of strychnine (0.5 µgin 0.25 µlaCSF) within the superficial laminae of the MDH (n = 7) significantly increased the firing responses of Sp5O WDR neurons to brushing (Figure 3).
For tracing experiments, cholera toxin B (10 µg/ul, 1 µl, Invitrogen) was injected into the CA3 region at a rate of 0.2 µl per min using a micro-infusion pump three days before sacrifice.
Micro-infusion of strychnine (0.5 µg in 0.25 µl aCSF) in the Sp5O significantly increased firing responses of WDR neurons to brushing (n = 9), without altering their basal firing and this effect lasted for 29±3 minutes (Figure 2).
Group 1 will receive placebo treatment via an implanted micro-infusion pump for three months.
In contrast, micro-infusion of ANI into the hippocampus or mPFC did not affect additional training.
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