For W = 1.5 μm, we can still observe the strong position dependence; P 1 and P 4 reflect two distinct micro-domains, and the other points can be considered as mixtures of such micro-domains.
From these results, we therefore confirm that nano-domains compose a single micro-domain and contribute to the SH response coherently, whereas micro-domains contribute to the SHG response incoherently.
The more loosely arranged interfibrillar region in β-PP matrix is capable of accommodating OBC hard blocks and inducing finer dispersion of OBC, which may lead to fewer OBC crystallization nuclei in each OBC micro-domain, and consequently the slower crystallization of OBC in β-PP matrix.
Ethanol affects MOP mobility and the plasma membrane lipid dynamics, hinting at MOP release from micro-domains and redistribution at the plasma membrane.
From the nanosclae imaging standpoint, we sought to determine whether TCR/CD3 ligation also led to CD3-CD8 co-clustering in the same nano- or micro-domains, and whether CD28 co-activation could also enhance CD3-CD8 co-clustering aseenen for CD3-CD4 co-clustering.
In fact, the percentage of CD8 recruited to the nano- or micro-domains and CD8 molecular density in the nano- or micro-domains were much lower than their CD4 counterparts after anti-CD3 Ab and anti-CD28 Ab co-stimulation (Fig. 4E, 4F, p<0.05).
Other cutting-edge technologies like single-particle tracking in living cells [ 28] will allow the development of more realistic models, and will enable the investigation of the role of micro-domains and supra-macromolecular complexes.
Studies now suggest promiscuous protein protein interactions (PPIs) between β-barrel OMPs in Escherichia coli govern their local and global dynamics, engender spatiotemporal patterning of the outer membrane into micro-domains and are the basis of β-barrel protein turnover.
Many of these proteins are lipid raft-associated and may cluster to form membrane micro-domains, and in turn recruit multi-protein complexes functioning in membrane trafficking and signal transduction [ 26].
Recent work by Katsumata et al. (2007) has shown that the secretory granule membrane is composed of two kinds of micro-domains and that the proteins expected to be removed during the membrane remodelling (VAMP4 and Syntaxin6) are clustered together in the same micro-domain and separated from the proteins (VAMP2) that stay on the MSG membrane.
