The micro-chambers are fabricated with polyanhydrides (CPP-SA) using the UV-LIGA technology and the controlled release process are the combined results of the design of the micro-chambers and the biodegradable characteristics of the polymer.
The micro-chambers were fabricated with biodegradable polymer using the UV-LIGA technology and the controlled release process is the combined results of design of the micro-chambers and the biodegradable characteristics of the polymer.
Chemotaxis of neutrophils was measured with 48-well micro-chambers and polycarbonate filters (5 μm pore size) (NeuroProbe, Cabin John, MD) as described (Tancevski et al., 2014).
By using pressure from dual micro chambers, the Nomad can apparently achieve the same results as a full-size lever espresso machine.
The procedure when and how the liquid was lowered was the same for each figure with micro chambers, except from the figures where it is indicated that "The buffer was not yet reduced to a level below the upper level of the chip".
The mixture of the aquarium microbes and atoll water was placed in micro-respirometry chambers and the oxygen concentrations were measured using micro-oxygen sensor probes (Unisense, Aarhus C, Denmark).
Monolayers of HBE cells were mounted in micro-Ussing chambers and analysed under open-circuit conditions at 37 °C, as before (Moniz et al., 2013).
Freshly removed liver and lung tissues were dissected into thin slices of approximately 2 mm in thickness and immersed under PBS in a micro chamber on a glass slide for examination.
The biodegradable PCL micro chamber was prepared using the x-ray-lithography-based microfabrication method [20].
Cells then were mounted into a perfused micro Ussing chamber and the luminal and basolateral surfaces of the epithelium were perfused continuously with ringer solution (in mM: NaCl (145), KH2PO4 (0.4), K2HPO4 (1.6), glucose (5), MgCl2 (1) Ca-gluconate (1.3)) at a rate of 6 ml/min (chamber volume 2 ml).
