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ED carried out the micro array, tissue array and RT-PCR experiments, designed the study and drafted the manuscript.
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We used tissue micro array with a tissue core diameter of 2.0 mm, but although this is comparatively large we do not know whether the degree of heterogeneity of YKL-40 protein expression in OC tissue could have influenced our results.
Formalin-fixed and paraffin-embedded cancer tissues were sampled in a tissue micro array with cores of 2.0 mm (Manual Tissue Arrayer 1, Beecher Instruments, Silver Spring, MD, USA).
The amount dissected was determined both by the length of cancer available in the core, and also to enable preservation of any remaining cancer tissue for tissue micro array studies.
The ACSR provides Tissue Micro-Array (TMA) tissue sections with de-identified clinical data to approved researchers.
This tissue micro array LC242 represents 10 cases of lung tumor with 2 non-neoplastic tissue (duplicated core in 1.5 mm size per case) sections.
The tumour areas were identified and sampled in triplicate with a 0.6 mm diameter tissue array punch (Beecher Instruments Inc., Sun Prairie, WI, USA) to create a tissue micro array.
Formalin-fixed paraffin-embedded tissue was available from 93 of the 101 patients as part of a Tissue Micro Array (TMA).
Adjacent normal breast tissue did not stain for either GRP78 or XBP1 in keeping with the findings of the tissue micro array.
The specimens were used to construct a tissue micro array (TMA) using a Beecher Instrument (Sun Prairie, WI, USA).
The Tissue Micro Array were constructed from surgical specimens obtained in the Institut Claudius Regaud, the patients gave a general written informed consent and the use of these specimens was authorized by the IRB of the Institut Claudius Regaud.
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