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Tissue micro array sections were dewaxed in xylene and rehydrated in ethanol before microwave antibody retrieval.
Nuclear area, form factor and fractal dimension of chromatin texture were obtained from digitalized images of hematoxylin-eosin stained tissue micro array sections.
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Formalin-fixed tissue micro array (TMA) sections were deparaffinised, rehydrated and washed.
To this end, we examined through immunohistochemistry the GM3 and phosphorylated STAT3 (pstatus status of 176 primary NSCLC sections in a tissue micro array (TMA) and correlated this to DC infiltration, microvessel density and overall survival.
It requires the use of optimally cryopreserved or fresh tissues as well as DNA micro array technology which is more costly than immunohistochemistry on paraffin sections.
In order to validate the micro array data, a total of 23 transcripts were quantified using qRT-PCR as described in materials and methods section.
This tissue micro array LC242 represents 10 cases of lung tumor with 2 non-neoplastic tissue (duplicated core in 1.5 mm size per case) sections.
Strong correlation between PCR expression data and micro array values was found (Figure 3).
Tissue micro array (TMA).
BG carried out the micro array protocol.
SSC reviewed the pathology, constructed the micro array and assessed the micro array staining.
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