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For a more detailed analysis, micro-array investigations were performed on myocardial tissue 4 h after pre-conditioning as well as 30 min and 3 h after reperfusion.
Using RNA obtained for the mice employed in the micro-array investigations in study 2 we validated findings for 4 genes (Ccl4, Ccl8, Il6 and Mmp2) using real time PCR.
Boyko: In a wet lab, we use what's called a micro array.
Strong correlation between PCR expression data and micro array values was found (Figure 3).
Tissue micro array (TMA).
BG carried out the micro array protocol.
SSC reviewed the pathology, constructed the micro array and assessed the micro array staining.
Micro Array reagent systems (Corning Inc., Corning, NY, USA).
LZ was involved in the Tissue Micro Array production.
The results were coherent with the micro array results.
Arrays were scanned with an Agilent G2565AA micro array scanner (Agilent technologies).
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