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We performed micro array analysis of whole animals and SMA affected tissues across normal and smn mutant animals.
The increased expression of Acadm, Gpx3, Gpx1 and Ndufa3, identified by the micro array analysis, was confirmed by q-PCR (Table 2).
The transcriptional changes were examined by micro array analysis using a 17 K Atlantic salmon cDNA microarray.
These included: chromosome micro array analysis for copy number variants (CNVs) (Affymetrix Genome-Wide Human SNP Array 6.0); telomere length analysis; CT and MRI scans and comprehensive metabolic testing.
The cDNA-AFLP allows an unbiased screening of the genome and is not restricted to genes that are available in the public databases as is the case for micro array analysis.
In contrast, micro array analysis performed at 48 hpf reveals the opposite pattern: Genes down-regulated at 24 hpf are up-regulated at 48 hpf in the presence of As3+.
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Patients will be reassessed with targeted ultrasound and magnetic resonance imaging (MRI) and will be offered genetic testing (today this is by micro-array analysis) [9].
The main focus of this review is on key biological concepts that can be accessed by chemostat-based micro-array analysis.
After a brief introduction to chemostat cultivation and micro-array analysis, key aspects of experimental design of chemostat-based micro-array experiments are discussed.
In this case, more than 200 genes are up-regulated as indicated by micro-array analysis, and one third of these genes, besides genes related to defense, are related to metabolism, transport, proteolysis, and signal transduction.
The most recent technologies such as micro-array analysis, though cost effective, provide estimates of gene expressions for thousands of genes simultaneously and need attention by the molecular biologists.
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