LC-MS/MS analysis was performed at the Génome Québec Centre of McGill University (Montréal QC, Canada), using a Q-TOF micro apparatus (Waters, Milford MA, USA) and a nanosource modified with a nanospray adapter (New Objective, Woburn MA, USA) to hold a PicoFrit column (BioBasic C18 packing, 5 μm, 300 Å).
An aliquot of 10 ml is taken from the digested sample and reacted in micro Kjeldhal apparatus with 10 ml of 40% NaOH to produce ammonia.
Grain samples (around 200 g) were micro-malted in a Micro-malting Apparatus (Phoenix System, Adelaide, Australia) using the following regime: 6 h steep, 14 h air-rest, 8 h steep, 14 h air-rest and 4 h steep, followed by 96 h germination – all performed at 15°C.
In FTIR, samples of neat epoxy and epoxy incorporating MWCNTs were analyzed with biconical reflectance Micro-Fourier Transform Infrared Spectroscopy (Micro-FTIR) apparatus.
Grain samples were micro-malted in a Phoenix System Micro-malting Apparatus (Adelaide, Australia) in the order of steeping, germination and drying.
Laser micro-dissection was performed using the PALM MicroBeam micro-dissection apparatus (PALM Microlaser Technologies).
The extraction experiments were carried out in a micro-device apparatus designed for high-pressure processes, in which the micro-device is the unit where the solvent and the liquid feed come into contact.
Shrinkage temperature of the unprocessed HAM and DHAM were measured using micro-shrinkage apparatus.
Moisture content was estimated by drying the samples to a constant weight at 85°C in a drying oven (GCA, model 18EM, Precision Scientific Group, Chicago, IL, USA), and nitrogen content, using a micro-Kjeldahl apparatus (Labconco Corporation, Kansas, MO, USA).
Experiments on the continuous extraction of ethanol from aqueous solutions using supercritical CO2 as solvent were performed to study the feasibility of the extraction process in a micro-device apparatus.
The variability of film crystallization in many regions of the samples has been studied, by using as heating agent the same laser beam of 632.8 nm of the micro-Raman apparatus.
