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Total RNA was extracted from brain and muscle tissues using the Qiagen RNeasy Micro Kit, as above.
Total RNA pooled from two samples per condition was subsequently extracted using the RNeasy micro kit as recommended (Qiagen).
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Total RNA from liver was extracted with the Qiagen RNEasy Micro kit (GERMANY) as described in the manufacturer's instructions.
The aqueous layer containing the RNA was diluted 1 1 with 70%% ethanol and purified using the RNeasy micro kit (Qiagen) as per the supplier's instructions including the optional on column DNase digestion step.
Lung RNA was extracted using an RNeasy Micro Kit (QIAGEN, UK) as per the manufacturer's instructions.
Total RNA was extracted from fibroblasts using Trizol (Invitrogen, Carlsbad, CA, USA) and from brain tissue using the RNAeasy Plus Micro Kit (QIAGEN, Venlo, Limberg, Netherlands) as per manufacturer's instructions.
Total RNA was isolated using the RNeasy Micro kit including DNase digestion as suggested by the manufacturer (Qiagen, Hilden, Germany).
For PML-RARα DNA real-time PCR, DNA was extracted using QIAamp DNA Micro kit (Qiagen) and quantified as previously described (Nasr et al, 2008).
Cells were washed with PBS then lysed and RNA was purified with the RNEasy Plus micro kit (Qiagen Inc., Valencia, CA) as directed by the manufacturer.
RNA was then extracted with the RNeasy micro kit (Qiagen,CH) and cDNA generated as previously described [56].
Total RNA from SLK cell lines was prepared using an RNeasy Micro kit (Qiagen, Valencia, CA, USA) as per the manufacturer's instructions.
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