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A single Pink1+/− mouse showed a value of 0.52 (63% of wildtype), while Pink1−/− mice yielded an average signal of 0±0 (n = 6).
Similar studies in F3 mice yielded an overlapping survival curve.
As expected, the RT-PCR product of RNA isolated from wild-type C57BL/6J mice yielded an normal cDNA fragment of 400 bp.
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However, interbreeding of heterozygous mice yielded a sub-mendelian ratio of genotypes of offspring (+/+: +/−: −/−; males: 61∶103∶35; females: 43∶84∶8).
DNA from wild-type mice yielded a 750 bp band, while floxed mice were identified by the presence of a 650 bp band.
As expected, after BamH1 digestion, the WT mice yielded a single band of 7 kilobase pairs (kb), and the Grp94+/− mice yielded two bands (7 kb and 6 kb) of equal intensity (Figure 1B).
Pancreatic islets from dox-induced double transgenic mice yielded a FRET signal when excited at 430 nm, and more importantly, showed a change in FRET intensity when intracellular cAMP was elevated experimentally.
Finally, the Brucella-susceptible BALB/c mice yielded a larger span between control groups (PBS [150 mM NaCl, 7 mM KH2PO4, 10 mM K2HPO4; pH 6.85]- and Rev 1-inoculated mice) and more homogenous results than CD-1 mice, thereby allowing better statistical evaluations (see Figure S3).
Adoptive transfer of HuD p321-specific CD8 T cells together with DC pulsed with irrelevant peptide into Rag−/− mice yielded a lower level of in vivo cytotoxicity (20%; data not shown), suggesting that the DC pulsed with p321 served to expand the adoptively transferred population of HuD specific CD8 T cells in recipient mice.
Between 50 and 75% of the mice yielded a palpable primary xenograft tumor.
Nevertheless, the fact that crossing two NAR IIJ mice yielded a similar incidence of arthritis suggests that the trait is also incompletely penetrant, since the NAR parents must harbor the disease-promoting alleles but do not display the trait.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com