The goals of this study were: 1) to determine if high-fat diet (HFD) feeding in female mice would negatively impact biomechanical and histologic consequences on the Achilles tendon and quadriceps muscle; and 2) to investigate whether exercise and branched-chain amino acid (BCAA) supplementation would affect these parameters or attenuate any negative consequences resulting from HFD consumption.
To determine whether Pneumocystis-induced macrophage polarization would impact subsequent immune responses, we infected mice with Pneumocystis and then challenged them with Pseudomonas aeruginosa 14 days later.
In order to investigate whether inhibition of p110α would impact on the development of cancer driven by PTEN loss, we crossed PTEN+/− mice with mice heterozygous for the p110α kinase-dead D933A knockin allele [ 28].
We next injected the clones Tm1G3 or Tm1N3 into WT or KO mice to address whether the source of galectin-3 (tumor vs stromal origin) would impact on tumor engraftment and growth.
We also examined 10-day 4T1 tumors in mice that were treated the last three days with dovitinib, to see if blocking FGFR would impact on activation of other RTKs.
To examine whether the absence of p21cip1 or p27kip1 would impact SC repopulation and differentiation in vivo, we performed repeated muscle injuries on Ctrl, p21 null and p27 null adult mice (Fig. 8D-I).
Lastly our conclusion that both functional domains are necessary to cause phenotypes in the duplication mice would not be impacted by the outcome of endogenous MeCP2 IP.
In particular, analysis of neuronal mRNA/ protein levels and distribution for ENTPD1, 2 and 8 in ENTPD3 knockout mice would increase the impact of the findings reported here.
To optimize the number of days mice would be on study diet, we assessed impact of diet administration for varying lengths of time, monitoring mouse body weight and condition.
To examine whether the buffering impact seen in the Robey et al (2009) study in mice would be achievable with the same equivalent dose in humans, we simulate the buffer therapy with human parameters and translate the bicarbonate dose.
