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Consider how toxicology has exploited the tools of contemporary molecular biology to create novel organisms such as mice with unique genetic properties.
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Any individual mouse, with its unique ID, could be traced back to its lineage, body weight or neurological score on any given day, and any other observations noted during its lifetime.
We have constructed a physical map and produced experimental patterns of mouse γ-satellite DNA hydrolysis with unique site-specific methyl-directed endonuclease GlaI and several restriction endonucleases.
Probing of human, mouse and rat RefSeq databases with unique query contigs typically produced matches to the same transcript in each library thereby improving annotation fidelity of hamster transcripts.
To mimic evolving human tumors, we transplanted a mixture of the parental B2M +/+ and the B2M-deleted cell lines (each tagged with unique markers) into mice in the presence or absence of NK cells (using antibodies to deplete NK cells) (Supplementary Fig. 17c).
Mice with a naturally occurring mutation in the BAFF-R locus (A/WySnJ mice) have a qualitatively similar phenotype to mice with BAFF deficiency, suggesting a unique role of BAFF-R in B-cell development that cannot be compensated by the two other BAFF receptors, TACI and BCMA [ 9, 10].
In summary (Fig. 9), these data indicate that all Tg and KO mice with disrupted collagen X function have unique skeletal and hematopoietic defects that arise from collagen X disruption in hypertrophic cartilage.
The role comes with unique challenges.
* With unique coordinates.
Experiment with unique angles.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com