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This question was addressed by analyzing mice with simultaneous inactivation of both Notch1 and Notch2 in the epidermis.
Complete elimination of Notch activity could be achieved through the generation of mutant mice with simultaneous deletion of the three bHLH-O genes hes1, hes3 and hes5, which encode the main effectors of Notch signalling in the embryonic spinal cord [6], [9].
This model has been shown to yield similar results to the DTH model.[19] DTHR was induced in the pinnae of naïve C57BL/6 mice with simultaneous injection of subcellular DBA/2 alloantigen (35 µl) and splenocytes (8×106cells/condition) harvested from these sensitized "rejector" mice between 30 60 days post transplant.
Transgenic mice with simultaneous ablation of TGFβ-signalling and reduction of FOXG1 in neural progenitors and neurons suffer from ICH mainly in the forebrain.
As reported by LeBoeuf et al (2011), mice with simultaneous deletion of Hdac1 and Hdac2 (Hdac1Δ/Δep Hdac2Δ/Δep) were not viable confirming an indispensable role of HDAC1 and HDAC2 for epidermal development (Supplementary Figure S1L).
Thus, we next tested the sensitivity of CaRuby-Nano using in vivo patch-clamp recordings from neocortical layer 2/3 pyramidal neurons in anesthetized mice with simultaneous two-photon [Ca2+] imaging (Svoboda et al., 1997).
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Docetaxel followed by castration improved outcomes in LNCaP PC-bearing mice, compared with simultaneous therapy (Tang et al, 2006).
In this study, we found that TCP-1 β overexpressed in the tubules of kidney in type 2 DM mice with a simultaneous rise in Ccr.
Here, we established a conditional mouse model with simultaneous S100a8 and S100a9 transgene expression in hepatocytes (TgS100a8a9 hep ) under the control of doxycycline to unravel the role of epithelial-derived Calprotectin on tissue homeostasis and inflammation.
Through use of a mouse model with simultaneous activation of oncogenic KRAS and inhibition of GLI1 in the pancreas epithelium, this group also demonstrated that decreased GLI1 activity reduced the incidence of KRAS-driven PDAC precursor lesions (pancreatic intraepithelial neoplasias or PanINs) and PDAC.
Forepaw dyskinesia with turning steps: time mice spent in forepaw dyskinesia with simultaneous stepping movements of the hind paws in one direction, resulting in a combined rearing turning and rapid forepaw movement along the cylinder wall (Movie S2).
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