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Control mice with mixed backgrounds will also become fixed, but for different B6 and 129 alleles than the KO strain.
We applied this technique to the generation of congenic strains from mice with mixed genotypes bearing a transgene, a targeted KI gene or chemically induced mutant genes.
PPARγ+/+ and PPARγ−/− TS cells were derived from E3.5 blastocysts from PPARγ+/−×PPARγ+/− matings of mice with mixed (C57Bl/6J 129 FVB) genetic background [26], [27], on murine embryonic feeder cells (MEF), and cultured as previously described [14].
This may be due to the different genetic backgrounds between cell lines, similar to the observation shown in Figure 4C that primary cells from B6 and Balb/c are less infectable than cells from transgenic or knockout mice with mixed background.
While most of these experiments have been performed in mice with mixed genetic backgrounds or congenic with the 129/sv strain, one study found that Per2−/− mice congenic with the C57BL/6J strain have periods of wheel-running activity that are indistinguishable from wildtype mice and they do not become arrhythmic in DD [33].
However, it should be noted that mice with mixed background were used for Trp53 and Cdkn1a rescue experiments.
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Similar to our previous seed mouse (TOKMO-1: Roseed seed mouse with mixed genetic background of C57BL/6J and 129/Ola), both homozygotes and heterozygotes of the new seed mouse (termed TOKMO-3, line BDT 73) were viable with normal reproductive ability.
For in vivo osteogenic evaluation, PKH26 labeled hMSCs were implanted into the subcutaneous spaces of athymic mice with a mixed scaffold.
In vivo angiogenesis in mice with plugs mixed with bFGF/2-DG bFGF/2-DGficantly less neovascularization, compared to thadcontrol group (Fig. 7A. I).
The IEC-specific deficiency of PPAR γ in mice with a mixed background worsened colonic inflammatory lesions, but had no effect on disease activity (DAI) or weight loss.
For analysis, heterozygous mice with a mixed FVBN/129SV (25% FVBN:75% 129SV) was used.
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