Impaired osteoclast activity is another reason for increased bone mass in mice with Fgfr1-deficient in differentiated osteoblasts.
At 1 μM concentration of incubation with differentiated mdx mouse cells in the presence of 10% FBS, but in the absence of any transfection agent, we were able to obtain >70% exon skipping in this model system, higher than for previously studied linear RXR peptide−PMO constructs (data not shown).
In cultured adipocytes, global deep sequencing identified 16 and 12 miRNAs that were differentially regulated in mouse primary pre-adipocytes when compared with differentiated mature brown adipocytes in vitro, [ 18].
As an example, conditional mice with Tsc2 gene inactivation in differentiated astrocytes have been shown to display a more severe phenotype than those with Tsc1 deletion (Zeng et al., 2011).
FACS-sorted CD62LhighCD44low naïve CD4+ T cells from IL-23KO mice or WT mice were differentiated to Th2 cells with splenic APCs from IL-23KO or WT mice and cytokine expression were assessed.
After 3 weeks of in vitro culturing, the hybrids with differentiated chondrogenesis were then injected subcutaneously into nude mice and retrieved after 4 weeks of culturing in vivo.
Moreover, mice with dominant negative erbB4 have more differentiated oligodendrocytes, but each of them seems to myelinate less axonal surface and to produce thinner myelin sheaths than its wild-type counterpart [ 159].
Additionally, when co-injected with SUM159PT mammary adenocarcinoma cells in athymic nude mice, fully differentiated 3T3-L1 adipocytestimulatetumormor growth and lung metastasis [ 42].
Myoblasts were isolated from wildtype and XIAP-deficient mice and differentiated in culture for 14 days prior to injection with cytochrome c.
The scaffolds were then implanted into dorsal skinfold chambers of C57BL/6 wild-type mice to analyze their vascularization and incorporation using intravital fluorescence microscopy, histology and immunohistochemistry. Scaffolds seeded with differentiated spheroids exhibited a markedly impaired vascularization.
