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Lexicon has published a full analysis of the retinal defects in these KO mice, with data on body weight and length and cartilage histologic abnormalities.
We performed a two-class test between results obtained from mock-infected mice and scrapie-infected mice, with data input parameters kept at the default values.
To obtain a profile of global transcript abundance in islets, we compared whole-genome expression data from islets of 8-week-old CHF-fed mice with data obtained by hybridisation of RNA from total pancreas of matched animals.
Throughout the analysis, 'mouse' was taken as the unit of analysis and only data from mice with data from both day 14 and day 21 or day 28 were included, because we looked at change over time in terms of proliferation, apoptosis and CTIs.
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For building and testing classification models, only data from individual mice with complete data – i.e. all 15 molecular features – were used.
Immunofluorescence analysis of these brain slices confirmed that immunoreactivity for KCa3.1 was much less evident in IPTG-treated mice, consistent with data obtained in vitro.
Few NCR+ ILCs were present in the colon of TRUC mice, consistent with data from other groups reporting a requirement for T-bet in NKp46+ ILC development and differentiation.
In analyses of individual parameters, 2 additional mice with relevant data are included.
Similarly, FAM-VAD-fmk staining cells were observed after in vivo treatment of tumor-bearing mice with cyclophosphamide (data not shown).
The histological findings were similar to the β-cell hyperplasia observed when PyMT was induced postnatally in Pdx1-tTA; tet-o-PyMT-IRES-Luc mice and in RIP7-rtTA; tet-o-PyMT-IRES-Luc mice with doxycycline (data not shown).
We also compared FoxP3+Treg numbers in the spleen and were unable to see any differences in IL-10-/ B cell mice compared to WT B cell mice with CIA (data not shown).
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