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Intraperitoneal inoculation of Balb/c mice with appropriate titers of RRV within the first 48 h of life leads to an initial viremia which clears later, but the biliary epithelium remains injured [ 44, 57, 102].
Mice with appropriate size of tumors were divided randomly into three groups including control group, ellagic acid low-dosage group (50 mg/kg/day), and ellagic acid high-dosage group (100 mg/kg/day).
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MEFs were cultured in MEF media and derived at embryonic day 14.5 from timed matings of mice with the appropriate genotypes (see below).
Cre expression may be induced virally [ 49] or in a tissue-specific manner by crossing the mice with an appropriate strain expressing a Cre recombinase transgene under the control of a promoter specific to the tissue of interest [ 48].
All mice were studied with appropriate littermate controls.
BACE1 −/− and BACE1 +/− mice were studied with appropriate age-matched littermate controls.
All KO and transgenic mice were studied with appropriate littermates of the three control genotypes.
All knockout and transgenic mice were studied with appropriate littermates of the three control genotypes in all studies.
The Australian Code for the Care and Use of Animals for Scientific Purposes was followed, and the mice were maintained with appropriate ethics approvals.
When a gaming mouse with the appropriate OS driver was used, all six PCs were able to measure RTs with an average error of less than 10 ms, which is comparable to the PVT-192.
Purified amplicons (~200 ug/ml) representing a full length mouse cDNAs together with appropriate controls, prelabelled landmarks and print buffer negative controls were added to an equal volume of DMSO and 10 ul transferred to 384 well plates.
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