All oral glucose administrations were conducted in a commercially available gustometer (Davis MS160-Mouse; DiLog Instruments), which permitted us to monitor the number of licks taken by each mouse.
The analytical capacity of these animal models might be expanded by genetically modified mice such as transgenes or knock-outs, which permit the understanding of disease-relevant processes.
Mouse extensor digitorum longus (EDL) muscles were dissected to small bundles of fibers, which permit measurement of sarcomere length (SL), by laser diffraction.
Ionophores are molecules which permit ion traversal of lipid membranes which are ordinarily ion-impermeable [23].
SNPs were genotyped in each mouse of the pedigree with the BeadArray technology (Illumina, San Diego, CA, USA), using the Mouse MD Linkage Panel, which permits analysis of 1449 SNP loci; bead chips were read in an iScan system (Illumina), as described [ 34].
To investigate whether centrosomes in myoblasts are capable of nucleating microtubules after differentiation, we used cultured mouse H-2Kb-tsA58 myoblasts, carrying a thermolabile T-antigen, which permits differentiation upon a temperature shift from 33 to 37°C [9] [10].
Imaging of living parasites within the intact spleen was achieved by placing the mice on a stage of a Leica TCS-SP5 microscope fitted with an inverted 63x (1.3 NA) glycerol objective, which permits a working distance of 0.28 mm.
The Rorα Cre mouse has an IRES Cre cassette knocked-in downstream of the Rorα locus (Chou et al., 2013) which permits normal expression of Rorα and co-expression of Cre.
Which permitted savagery and barbarism in its own great cities.
This modified pharmacokinetic behaviour permitted a higher drug exposure of the hydatid cysts developed in mice, which enhanced the clinical efficacy of FLBZ in echinococcosis [ 21].
