Furthermore, cytokeratin staining of tumors derived from M90TΔaroA-infected mice, which identifies pockets of 4T1 tumor cells showed reduced content of cytokeratin positive cells.
Accordingly, we examined the four myosin heavy chain genes, Myh1, -2, -4 and -7 (in wild-type and sg/sg mice), which identify and classify types I, IIa, IIb and IIx fibres, respectively (ESM Fig. 2a d).
This allowed design of mouse primers which identified a single positive clone in a bacterial artificial chromosome (BAC) library of mouse genomic DNA.
These results confirm and extend observations from a recent expression profiling study of age-associated gene expression patterns in cardiac tissue from multiple mouse strains, which identified both Pah and C4b as robust biomarkers of cardiac aging, and confirmed these age-related expression patterns using RT-PCR [ 11].
Human-mouse OR orthologs identification was based on a previous work, which identified human-mouse-dog three way mutual best hits [ 13].
In order to clone the mouse homologue of MCOLN1, the human amino acid sequence was compared to the high throughput genomic sequence (HTGS) database using TBLASTN, which identified the mouse BAC clone RPCI-23_387H4 (GB No. AC079544.1).
Autosomal recessive mutation derived diabetes in db/db mouse strain is spontaneously diabetic mice which was identified as a mutation in the leptin receptor gene [ 92].
This also agrees with comparisons of the crystallographic structures of the mouse s- and i-proteasomes, which identify only minor variations between the β2 and β2i catalytic pockets (Huber et al., 2012).
In the current study, we chose the model of APP/PS1 double Tg mice, which were generated by knocking identified familial AD and/or PS1 into the mice genome; this model has been widely adopted by other scientists.
