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Here, we studied the cerebellar gene expression patterns of young Sca6-MPI118Q/118Q KIockin (KI) mice, which expressed mutant Cav2.1 from an endogenous locus and recapitulated many phenotypic features of human SCA6.
These pathological features somewhat resemble those observed in the skeletal muscle of DM1 mice, which expressed expanded CUG transcripts [13], [14].
However, the mice which expressed relatively low levels of TWEAK (4 5 folds higher than littermate controls) survived and developed into adulthood.
The fact that CAG0 and CAG23 mice, which expressed high levels of EGFP protein, lacked a discernable phenotype further supports the notion that expanded CAG repeat RNA is deleterious.
Mammary tumours developed in each of the four independent lines of mice which expressed transgene IGF-II in the gland.
We developed human CD1 transgenic mice, which expressed group 1 CD1 molecules in a similar pattern to that observed in humans.
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Mice used were OT1 TCR transgenic mice, which express a TCR recognising the ovalbumin (OVA -derived peptide SIINFEKL, OVA -derivedransgenic mice, which expeptide TCR recogniSIINFEKL gp33-41 peptide or LCMV (KAVYNFATM).
To test this hypothesis, we established the Myf5 nLacZ/mdx mice by breeding mdx mice with Myf5 nLacZ mice, which express nuclear-localized β-galactosidase in Myf5-expressing cells (Christov et al., 2007).
The Ctgf Lo/Lo mice are similar in Ctgf expression level to the Ctgf heterozygous knockout (Ctgf KO/+) mice which express 50% of wild type Ctgf.
Mice carrying loxP flanked genes have for example been crossed with Cre-transgenic mice which express Cre in a cell-type-specific or inducible manner [ 11, 12].
To date, researchers have used transgenic mice, which express the protein channelrhodopsin 2 that is sensitive to blue light.
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