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Spleen and lymph node exhibited a composition very similar to laboratory mice, which allowed direct comparison for evaluation.
Most experiments were performed on OSNs isolated from OMP-GFP mice, which allowed using the green fluorescence of the cells in addition to the morphology to ensure the cells were mature, intact OSNs.
The first immunodeficient mouse model of cancer was developed in nude mice, which allowed the growth of human solid tumors, after inoculation of 1 20 million cells.
In wild type, ERα-/ and ERβ-/ mice, a similar level of TT-specific swelling was observed in vehicle-treated mice, which allowed for further compound profiling in this model.
Such a requirement has been elegantly demonstrated by injecting I-T4 and I-T3 into wild type and Dio2−/− mice, which allowed the evaluation of the relative contributions of plasma and D2-generated T3 in regenerating muscles.
For this, we generated Ntf3 stop :Rosa26 tdTomato :Pou4f3/CreER T mice, which allowed identification of hair cells in which the Ntf3 overexpression transgene had been activated, based on tdTomato fluorescence.
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We established a novel RUPP model of PE in outbred ICR mice, which allows one to evaluate BP using tail cuff (Fushima et al. PLOS ONE, 2016).
The B16-F10 tumor cells are implanted subcutaneously in mice, which allows indirect evaluation of the in vivo permeation of 5-FU across mouse skin as well.
Mouse models are pivotal because most genetically engineered animal models are mice, which allow dissecting the impact of selected target genes in renal failure.
In addition to oncogene dependence, the development of the MTB mice, which allow direct targeting of the mouse mammary epithelial cells, allows for the study of other factors that contribute to mammary adenocarcinoma progression.
We used a floxed vector to establish conditional HSP60 transgenic mice, which allows studies on inducible and organ-targeted HSP60 expression in adult mice and is potentially useful in pursuing essential questions related to HSP60 in vivo.
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