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The humanized baby mice, when isolated, made whistles that had a slightly lower pitch, among other differences, Dr. Enard says.
Moreover, significantly reduced insulin secretion was observed in beta Rac1−/− mice when isolated islets were stimulated with glucose.
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These results are essentially consistent with previous studies that have reported an improved responsiveness of the pancreatic beta cells to glucose when isolated mouse islets were incubated with liraglutide [ 10].
When isolated from mice pulsed for 12 hours between day 2.5 3 post CTX injury, both CD34+/− populations had incorporated equally high levels of BrdU.
Validating the use of a transplantation paradigm in lieu of intact transgenic mice, previous studies have shown that when isolated PyMT tumor cells are serially regenerated as tumors in recipient mice the morphology and gene expression profiles are similar to the tumors found in the transgenic mouse [ 28].
Amastigotes were also covered by host cell membranes rich in LAMP when isolated from mouse embryonic fibroblasts (MEF) after 48 h of transient infection (Supplementary Fig. S3B and C, Fig. 5A).
Similar results were obtained when isolated adipocytes from mice incubated under the 5.5 and 25 mM glucose conditions were stimulated with 10 ng/mL TNF-α.
When isolated and injected intravenously into SCID mice with rhabdomyolysis-induced AKI, human CD133+CD24+CD106- cells engrafted into the nephrons, generating tubular cells [72].
Differently, 7-hydroxymitragynine, a minor constituent (2%) of M. speciosa, when isolated demonstrates a potent antinociceptive activity in mice [ 14].
Using multiple fluorescent Rosa26rbw/+ Ddx4-Cre germline reporter mice, Zhang et al (62) reported that DDX4-positive cells isolated from adult ovaries were not mitotically active, whereas they were when isolated from testes.
When isolating splenic CD4+ T cells from mice infected with the parasitic helminth Schistosoma mansoni, we observed a defined population of IFN-γ/IL-4 double-positive cells.
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