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In brief, peritoneal neutrophils from TGA-treated mice were washed and resuspended in medium lacking antibiotics.
To study the co-localization of HSC70 and P140 peptide staining, freshly isolated splenocytes from MRL/lpr mice were washed with PBS and incubated at RT for 45 min in PBS containing 25 µM biotinylated P140.
The mice were washed with phosphate-buffered saline and incubated for 10 min in a 0.1% toluidine blue solution.
Tumors obtained from control and Triphala treated mice were washed with cold PBS, minced and homogenized in above-mentioned lysis buffer.
To prepare the cell membrane lysates, osteoclasts from wild-type and CD44−/− mice were washed three times with cold PBS and removed from the plates by gentle scraping with cold PBS.
The A549 tumors taken from the NOD/SCID mice were washed with ice-cold phosphate-buffered saline (PBS) pH 7.4 to remove any remaining blood, and then dissociated by mincing the tissue with scalpels, followed by addition of DMEM medium containing 1 mg/ml collagenase I and incubation for 60 90 min at 37 ℃.
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The peritoneal cavity of tumour-bearing mice was washed with 1 ml PBS to obtain peritoneal cells for FACS analysis.
In addition, the peritoneal cavity of tumour-bearing mice was washed with PBS and analyzed for tumour-derived PGE2 and NO production.
These organs from each mouse were washed by immersion in 0.9% saline solution and placed on the objective stage for imaging.
To count exflagellation events either 10 µl purified gametocytes were resuspended in 50 µl GAM or 3 µl of tail blood from an infected mouse were washed rapidly in 1 ml GMB, pelleted and resuspended in 30 µl GAM.
Hearts of neonatal mice and E17 mouse embryos were washed with PBS and fixed by 10% neutral formalin.
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CEO of Professional Science Editing for Scientists @ prosciediting.com