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Our in vitro and in vivo studies revealed that this strategy could boost the immune response to produce longer and stronger protection against target cells, and mice were thoroughly protected from tumor growth.
At sacrifice, all mice were thoroughly inspected for liver metastases.
Cardiac pathology and adaptation to HFD-induced obesity in the MRL mouse strain compared to the HFD C57Bl/6 mice were thoroughly analyzed with echocardiography, histology, qPCR, electron microscopy and immunoblots.
Mice were thoroughly examined and palpated for tumor formation, which consistently detected tumors as small as 4 mm diameter.
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69 The effect of TNP-470 on caloric intake and energy expenditure in high fat fed C57BL/6J mice was thoroughly assessed recently by White et al. The authors speculated that previous studies 69, 70 possibly overlooked the effects of TNP-470 on increased energy expenditure.
On completion of all four trials, the mice were dried thoroughly and returned to their home cages.
Heart tissues from control or drug-treated mice were homogenized thoroughly in RIPA buffer containing proteinase inhibitors [ 14].
For isolation of skin-resident cells, mice were shaved thoroughly and abdominal, dorsal and flank skin carefully excised.
To control for odor cues, the experimental cage was changed for each mouse, and the objects were thoroughly cleaned with 90% ethanol, dried, and ventilated for a few minutes between mice.
Mouse spleen cells (1 × 10) and cells (1 × 10) of the mouse myeloma cell line P3X63Ag8.653 were thoroughly mixed and cell fusion was mediated by polyethylene glycol 1500 (Boehringer Mannheim; Germany) [ 19].
Four, 8 and 48 weeks after I/R, mice were euthanized and the kidneys were thoroughly perfused with saline to remove blood from the vascular beds.
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