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The mice were subject to DMBA/TPA two stage carcinogenesis as described previously45.
After donor chimerism was stable over 90%, chimeric mice were subject to PHZ challenge.
In behavioral study 2, a new set of female mice were subject to OVX procedure followed by treatment of either control (CTRL; injection of sesame oil with 0.25% benzyl alcohol) or estradiol benzoate (EB).
(A E) The wildtype mice were subject to cold challenge.
(A D) The wildtype mice were subject to cold challenge.
(A and B) The wildtype mice were subject to cold challenge.
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Mice that were designated as 'sham' mice were subjected only to the craniotomy procedure after anaesthesia.
Male mice were subjected to social stress.
C57BL/6J mice were subjected to CSDS for 10 consecutive days, as described previously49,50.
At day E14.5 the pregnant mice were subjected to IdU injection.
After donor chimerism stabilized (~90%), transplanted mice were subjected to PHZ challenge.
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