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Mice were stopped after ≥5 consecutive seconds on the pulse grills.
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Daily trials lasted 60 seconds each and were stopped if the mouse reached the submerged platform where they were maintained for 15 seconds.
Measurements were stopped when one mouse in the cage reached maximum tumor size (> 1 cm in one of its dimensions) and had to be euthanized.
All treatments were stopped 30 days later, and the mice were kept under observation.
Tumour growth curves were stopped at the exclusion day of the first mouse in each group.
To address the question of whether cysts would repopulate the lungs of echinocandin- treated immunosuppressed mice after therapy was stopped, 2 studies were performed using only anidulafungin (1 mg/kg).
In addition, samples from an experiment where tumor-promoting chronic treatment of mice with phenobarbital had been stopped 2 weeks prior to killing (Bursch et al. 2005) demonstrate that the drug-metabolizing phenotype of the tumors is stable after withdrawal of the promoter.
When mice failed to find the platform within 90 s, the trial was stopped and mice were put on the platform for 10 s.
Treatment was stopped in mice when tumors reached 14 mm in diameter and then restarted in half of the mice after one week (the average time to increase from 14-16 mm).
After 28 days, treatment was stopped and mice were again followed for tumor recurrence, which eventually developed in about half of the mice.
Exercise was stopped when mice were unable to maintain running despite repeated contact with the rotating plastic strips.
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CEO of Professional Science Editing for Scientists @ prosciediting.com