For each experiment, the number of mice used is stated in the figure legend.
Kang et al. describe that mice 4 6 weeks of age were used for hydrodynamic delivery while in the outline of the reproducibility project it is stated that mice 4 6 weeks or 6 8 weeks of age are ordered and used after one week of adjustment time.
Bar charts plotting mean±SEM display the mean average and associated standard error of all samples/mice across all experiments performed, and the number of mice used to calculate mean±SEM is stated in figure legends.
Since expression of Cre recombinase does not result in lethality in other conditional mouse strains, and since the Jackson Laboratory currently has another Tek-Cre mouse strain in development, which is stated to be viable as Tek homozygotes, we suggest that this lethality originates from the site of insertion of the Tek-Cre transgene, which may disrupt the function of an essential gene.
Unless otherwise stated, mice were anesthetized and perfused with PBS 4 weeks after infection.
For primary systemic infections, unless otherwise stated, mice were i.v. injected with ∼1×104 LM via the lateral tail vein.
Proximal and distal colitis scores from individual mice were averaged, unless otherwise stated.
Unless otherwise stated, mice were immunized with 100 μg of p35 55 emulsified in complete Freund's adjuvant (CFA, Sigma, Poole, UK).
At the end of the 7-day period, mice were sacrificed and processed as stated above.
When stated, recipient mice were given 2 mg tilorone analogue T-8014 or T-7514 T-7514a-Aldrich, Stockholm, Sweden) orally one day before injection of donor cells.
