Briefly, spleen cells from DBA/2 mice were stained with 0.5 μM CFSE (CFSElow) and spleen cells from C57BL/6 mice were stained with 5 μM CFSE (CFSEhi).
Activated T cells from either wild type or FADD-D mice were stained with FADD antibody (green and pink, respectively) and DAPI (blue).
d, Microglia from wild-type or Cd22−/− aged mice were stained with the particular anti-CD22 clone (Ox97) used for immunophenotyping.
For Oil red O staining, ice-cold methanol-fixed frozen sections from mice were stained according to described protocols 24,25,31.
We euthanized cell-treated mice every week post transplantation and harvested the liver tissues for immunofluorescence assays (Fig. 4b) and the liver tissues of normal SCID mice were stained as control (Fig. S1d).
(B) Cryosections from contralateral and denervated TA muscle of WT and Gcn2−/− mice were stained with wheat germ agglutinin (WGA).
b Tissue sections from 7 days saline and S. pneumoniae infected mice were stained for fibrin (ogen) by immunohistochemical analysis (red stain).
b Lung tissue sections from saline and S. pneumoniae treated mice were stained with picrosirius to confirm tissue collagen deposition (red orange birefringence).
(A) Splenic cells from seven-week-old mice were stained with anti-CD45, anti-Ly-6C, anti-Ly-6G, anti-CD11b, anti-CD11c, anti-CD3, anti-CD4, anti-CD8, and anti-B220 antibodies, followed with flow cytometry (n = 4 5).
(b) Adjacent sections from TG APP23 (27 months), Tg2576 (22 months) and APPswe-PS1dE9 (19 months) mice and WT control mice were stained with specific anti-Aβ1-40 antibody mo, age of animal in months.
E10.5 and E12.5 mice were stained with Hoechst or propidium iodide respectively.
