Sentence examples for mice were snap from inspiring English sources
Brains from Hoechst/Evans blue-injected mice were snap frozen in TissueTek in a dry ice/isopentane bath.
Livers from infected mice were snap frozen in isopentane, embedded in OCT and stored at −70°C until use.
Luciferase activity in transfected muscle was determined using the luciferase assay system (Promega Corp .. Quadriceps muscles dissected from treated mice were snap frozen and stored at -70°C.
Briefly, excised pieces of skin from mice were snap frozen in liquid nitrogen and homogenized on ice in 500 μl of PBS with 0.01 M EDTA and a proteinase inhibitor mix (Sigma-Aldrich, Poole, UK) and 1 ml of 1.5% Triton X-100 in PBS.
The gel pieces retrieved from the mice were snap-frozen in liquid nitrogen, ground in a mortar, then dissolved in RLT-buffer for RNA extraction.
Kidney tissues from 12-month-old Cicf/f and Cicf/fVav1-Cre mice were snap-frozen in OCT medium (Sakura Finetek, 4583), then cut into 10-μm sections (Leica CM3050S).
Grafted RA synovium samples from SCID mice were snap-frozen.
Tissues from mice were snap-frozen in optimal cutting medium (TissueTek; Sakura, Torrance, CA, USA).
The brain tissues from the BALB/c mice were snap-frozen by immersion in isopentane chilled to −70°C, and the tissues were then immediately mounted in OCT medium.
Freshly isolated pancreas tissue from 12 14 week old Idd9 congenic and NOD mice was snap frozen in OCT medium (CD8 staining) or fixed in 10 % NBF and paraffin-embedded (TNFR2 and TRAF2 staining).
Tissue samples (liver, brain, lung, spleen, heart and intestine) were harvested from wild-type and Xiap −/− C57BL/6 mice and were snap frozen in liquid nitrogen and stored at −80°C until required.
