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The mice were singly housed in a temperature-controlled room at 27°C for a survival period of 10 days.
Behaviorally screened mice were singly housed in running wheel-equipped cages (wheel diameter 16 cm) under LD for at least 7 days then transferred to constant darkness (DD) for at least 14 days before cull.
For experiments assessing wheel-running activity, mice were singly housed in cages (33×17×14 cm) with unlimited access to a running wheel (diameter: 11 cm), food, and water.
Twenty-six male C57BL/6J mice were singly housed for several days then surgically implanted with iButtons (Maxim Integrated Products) [52], which were programmed to record core body temperature rounded to the nearest 0.0625°C at 15 minute intervals for a period of 35 days (day -7 to 28).
Control mice were singly housed as well.
All mice were singly housed and received water AL.
Similar(27)
The mice were singly-housed in standard (type IIL) mouse cages under standard colony conditions, and provided laboratory rodent food (Altromin rodent diet 1324) and water ad libitum.
TRPV4 WT and KO mice were singly-housed from the time of radiotelemeter implantation throughout the duration of the study.
Mice were singly-housed, had free access to food and water and were maintained on a 12 h light/dark cycle (lights off at 1900 h) at a temperature of 19 21°C and 50% humidity.
Each mouse was singly housed on arrival and was given at least 1 week to acclimate before testing.
There were infrequent circumstances (∼5 mice total in these studies) when a non-clamp study mouse was singly-housed (for example, an aggressor had to be separated from cage mates to prevent stress and injury to the other mice and to ensure access to food. Mice were housed under controlled light (12∶12 light∶dark) and climate conditions with unlimited access to food and water.
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