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For the diet experiments, mice were single housed at 10 weeks of age.
Female C57BL/6J mice were single housed for one week, after which they were given one hour access to a female C57BL/6 Tyr partner (phenotypically white C57BL/6J mice which can be more easily discriminated during manual annotations) from ZT 9 to 10 in their home cage for 28 days consecutively.
Pregnant female mice were single dosed subcutaneously with 4 mg of TM at the specified embryonic day.
14 days before the behavioural experiments, mice were single housed under a reverse 12 h : 12 h light-dark cycle (lights on: 21 00 h) in standard laboratory conditions (22 ± 1°C, 55% humidity, food, and water ad libitum).
After 2 weeks, mice were single housed in TSE PhenoMaster/LabMaster feeding/drinking monitoring cages (TSE Systems, Bad Homburg, Germany) and acclimatised for a week further before the start of 1 week of baseline food intake and locomotor activity measurements (phase 1).
Mice were single housed and given one week period of habituation, after which half of the cages were equipped either with a functioning or blocked wheel.
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In the primary screen mice were single-caged and fed ad libitum for a period of 14 days.
At the age of 8 weeks mice were single-housed in Macrolon type III cages and 1 week later a wheel was introduced into each cage, as reported previously [10].
The sciatic nerves from four mice were single-labelled with antibodies against neurofilament, NRG1 and ERBB2.
For homecage activity measurements, naïve mice were single-housed for 72 h in standard cages with food and water ad libitum.
The mice were single-housed in duplex caging in a room maintained at a constant temperature 20-222 °C) and humidity 30-700%) in a light:dark 12 12-h 12 12-he, according to escheduleed accordingotocols and NIH guidestablished
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