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The mice were shaved on their armpits and then a single injection of 1 × 106 B16 cells in PBS was administrated.
For cancer model preparation, all mice were shaved on their right armpits and then subcutaneously subjected with a single injection of 1 × 106 B16 cells in PBS.
In order to eliminate autofluorescence by the fur, mice were shaved in the abdominal and upper leg area and treated with Veet cream (local pharmacy) to remove remaining hair.
Anesthetized mice were shaved in the posterior dorsal area and cleaned with 70% ethanol.
Balb/c mice were shaved prior to study to expose skin on the back.
Mice were acclimated, caged in groups of five in a barrier care facility, and fed animal chow and water ad libitum and all mice were shaved and the dorsal skin was cleaned with ethanol before cells injection.
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A first batch of 8 mice was shaved on the lower back (1 cm2), local anaesthetic cream (lidocaine and prilocaine) applied, and a≈10 μL fragment of primary human colon adenocarcinoma tissue (from patient CR-IGR-034P, tumour collection CReMEC, Oncodesign, [22]) injected subcutaneously in an intrascapular position by trocar under isoflurane anaesthesia.
Before subcutaneous injection of Wnt3a/PBS liposomes, the back of the mice was shaved and cleansed with antiseptic Betadine.
The dark fur of the mice was shaved away in order to increase the sensitivity of signal detection.
The dark fur on the back and the head of the mice was shaved away in order to increase the sensitivity of signal detection.
The backskin of 7- to 9-week-old miR-203+/+ and miR-203−/− mice was shaved.
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