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Signals in control mice were set to 1. Mice were anesthetised with isoflurane and sacrificed by decapitation.
The mice were set out in groups of ten and infected with a strain of influenza.
More old C57BL/6 mice were set to mate but failed from plugging.
Timed matings of mice were set up and embryos harvested at day E13.5.
Accordingly, three groups of mice were set in this study.
In both cases, cohorts of Rip1-Tag2 mice were set up for survival study.
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The relative RNA level calculated as 1/2 n (n is the number of PCR cycles) with the calculated value for the mock-infected mice was set as 1.
To map the loci responsible for low TNFR1 protein levels in S mice, an interspecies backcross between female (C57BL/6 × SPRET/Ei) F1 mice and male C57BL/6 mice was set up and N2 backcross mice were generated.
By comparing the frequencies of different crypt clusters (Fig 3C and F), we found the induction frequency (resulting in monoclonal crypts) for WT mice was set by p 0 WT = 0.006 ± 0.002 per crypt.
The mouse is set to remote mode and polled at a regular interval to determine whether any button has been pressed or if the mouse has been moved.
For animals harvested from a DD condition (30 44 days in DD), activity onset of each Fx/Fx control mouse was set as CT12.
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